Український біофармацевтичний журнал № 6 (29) 2013

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V. О. Antonyuk
LECTINS: DISTRIBUTION AND FUNCTION IN LIVING ORGANISMS AND PECULIARITIES OF THE PROCUREMENT OF RAW MATERIALS
It has been suggested brief overview of personal investigations and scienti@ic data on the distribution of lectins in higher plants, true fungi and animals. Peculiarities of distribution of lectins in plant organs, changes of their activity during vegetation, quantitative content of lectins in various organs and families, their physiological role and peculiarities in harvesting raw materials are considered. Our attention was focused on differences in the harvesting of raw materials for puri@ication lectins from true fungi and organs of animals, on the possible relationship of carbohydrate speci@icity of lectins and their biological function.
Key words: lectins; distribution; function; plants; fungi; animals; raw materials 

E. V. Babiy, K. F. Vashchenko
BIOPHARMACEUTICAL RESEARCH OF TILORONE RELEASE FROM OINTMENTS ON THE HYDROPHILIC BASIS
It has been studied release of tilorone in in vitro studies from ointments on the polietilenoxide basis. The technique of spectrophotometric quantitative determination of tilorone in dialysate using supplements has been created. Evaluation of methods has been carried out on the criteria of accuracy, linearity, repeatability, what showed that this methods can be used in vitro by study of tilorone release
from ointment bases.
Key words: ointment; tilorone; dialysis; spectrophotometric analysis; validation

A. Yu. Brodska, A. L. Zagayko, L. V. Galuzinska
BIOCHEMICAL ASPECTS OF DEVELOPMENT OF METABOLIC SYNDROME IN WOMEN DURING PREMENOPAUSE
The experimental data obtained in the comparative evaluation of women during the premenopausal period according to the presence of metabolic syndrome (in terms of hormonal status, lipids and carbohydrate metabolism components) for elucidation of the mechanism of metabolic disorders. It has been found that changes in lipid proBile are associated with the balance of estrogens and androgens and mediated with insuline resistance.
Key words: pre-menopause; metabolic syndrome; visceral obesity; hyperandrogenism; insuline resistance; dyslipidemi

I. P. Bukhtiyarova
STUDY OF ANTIOXIDANT PROPERTIES OF RALEUKIN ON THE MODEL OF DITHIZONE DIABETES RABBITS
Current therapy is based on the study of diabetes a number of pathophysiological mechanisms of the disease. In recent years, there is a hypothesis, according to which universal mechanism involved in the major biochemical abnormalities induced by hyperglycemia, is oxidative stress, which combines the insulin resistance with dysfunction of pancreatic β-cells, and this proves the usefulness of antioxidants
in the treatment of diabetes. Given the presence in the recombinant receptor antagonist IL-1 raleukin defined in previous studies of hypoglycemic action of interest to study its antioxidant properties. The paper presents the results of an experimental study of the influence of receptor antagonist IL-1 raleukin the maintenance of primary and secondary products of lipid peroxidation and antioxidant activity of animals in the model of dithizone diabetes in rabbits. Determined that raleukin detected lived pronounced antioxidant effect, reducing the concentration of primary and secondary products of lipid peroxidation, increasing the activity of antioxidant system in animals with an absolute insulin deficiency. By normalizing effect on these indicators raleukin not inferior to the reference drug anakinra and more than metformin. The combination of antioxidant properties with positive effect of raleukin on the initial reaction of non-enzymatic glycosylation and glucose metabolism suggests the prospect of its application in the treatment of type 1 diabetes.
Key words: dithizone diabetes mellitus; primary products of lipid peroxidation; an antioxidant effect; raleukin

V. M. Коvalenko, G. І. Borshchevsky, А. К. Voronina
SAFETY OF THE DRUG EFIALTM SPRAY, PRODUCED BY “FARMAK”
The drug EKialTM, spray was developed by «Farmak» (Kiev). It contained peptide complexes skin of pig and phosphatidylcholine. Subacute toxicity study showed that the drug EKialTM with repeated applications in for 28 days at a therapeutic dose and Kive times the therapeutic dose did not affect the death of the animals and their integral parameters, and did not cause the abnormal changes of hematological values and histopathological alterations in the various organs of animals studied. The allergenic and irritative properties of EKialTM to the skin and mucous membranes have not been detected.
Key words: Efial; safety; allergenic and irritative properties.

О. І. Naboka, Yu. V. Voronina
STUDY ON HYPOTENSIVE ACTIVITY OF QUINOCARB ON THE EXPERIMENTAL MODEL OF ARTERIAL HYPERTENSION IN RATS
The results of study substance of quinocarb-derivated anilid quinolinic-2-carboxylic acid in“luence on arterial pressure (AP) and heart rate have been suggested while single injection for seven-days period to rats with an experimental arterial hypertension. It is appeared that quinocarb while single intragastric injection to the experimental hypertensive rats in a dosage of 10 mg/kg exposed signi“icant antihypertensive effect which went to its maximum point in 6 hours and equaled 13,6 % (-24,0 mm MC), and its intensity and length slightly exceeded antihypertensive activity of hydrochlorthiazide (8,7 %, or -15,0 mm MC). Veritable decreasing of the AP (17,1 %) under the in“luence of new substance while 7-days injection af“irms the existence in quinocarb as the experimental substance signi“icant antihypertensive effect, what was higher compared to the one after a single injection. 7-days-injection of as quinocarb, as referent preparations hydrochlortiazide and enalaprile did not have any in“luence on the rats heart rate.
Key words: anilids of quinolinic-2-carboxylic acid; arterial pressure; heart rate; quinocarb.

V. A. Rybak, L. N. Maloshtan
EXPERIMENTAL STUDY HYPOGLYCEMIC ACTIVITY OF PLANT EXTRACTS
It is installed the most severe hypoglycemic activity in thick extract of beans in 6 hours in intact animals and in animals with a glucose load after 30 and 60 minutes, which was close by the activity of the reference preparation metformin and prevailed over the extracts dogwood leaves and fruits of lupine.
Key words: thick bean extract; dogwood leaves; fruit of lupine; hypoglycemic activity.

N. Y. Asadullaeva, I. M. Ryzhenko
THE EFFECT OF EXTRACT OF ARTISHOKE LEAVES ON EMBRIOΠAND FETOGENESIS ON THE MODEL OF ALCOHOL PLACENTAL INSUFFICIENCY IN FEMALE RATS
Based on the research conducted it has been determined that extract obtained from artichoke leaves on the model of alcohol placental insuf‚iciency in pregnant female rats has fetoprotective effect. It was proved that investigational medicine and reference medicine solcoseryl on the model of alcohol intoxication contributed to improvement of fetal biometrics parametres. Extract obtained from artichoke leaves causes fetoprotective effect and has priority over the reference medicine solcoseryl. The results obtained allow to recommend this extract in the complex therapy of fetal growth retardation in women with alcohol placental insuf‚iciency.
Key words: alcohol placental insuf‚iciency; extract obtained from artishoke leaves; solcoseryl; fetoprotective activity.

O. O. Vashchenko
INVESTIGATION OF ACUTE TOXICITY OF MEDICATED NAIL LACQUER WITH UNDECYLENIC AND SALICYLIC ACIDS
This article is devoted to investigation of acute toxicity of a new drug product in the form of nail lacquer under the conditional name «Undesal» which contains undecylenic and salicylic acids as active pharmaceutical ingredients. It was established that drug product studied is practically non-toxic (V class of toxicity).
Key words: medicated nail lacquer; acute toxicity; pharmaceutical ingredients.

A. A. Stremoukhov
MICROBIOLOGICAL SCREENING COMPLEXES OF DIFFERENT TYPES OF GALL
This article presents the results of the study of microbial activity fractions bile animals. A substantial antimicrobial activity of the lipophilic complex bile towards Candida albicans has been founded. Availability and range of antimicrobial activity of the aqueous residue by means of the method «testobjects» showed bacteriostatic and bactericidal term effects of exposure during 20 minutes.
Key words: animal bile; lipophilic complex glycoproteins; antimicrobial activity; microbiological screening.

N. Yu. Bondarenko
NIFEDIPINE QUANTITATIVE DETERMINATION IN MEDICINAL FORMS USING KINETIC METHOD OF CHEMILUMINESCENCE ACTIVATION
The procedure of Nifedipine chemilumeniscence determination in model solutions of pure substance, «Phenihydin-Zdorovye» and «Nifedipine» tablets based on activation of luminol chemilumeniscence oxidation by hydrogen peroxide reaction was developed. For Nifedipine assay RSD≤3.9% (n = 5, P = 0.95), the limit of concentrations studied (LOQ) is 2 ng mL-1.
Key words: determination; luminol; Nifedipine; kinetic-chemiluminescence method.

І. L. Komarytskiy, N. Yu. Bevz, V. А. Georgiyants
VERIFICATION METHODS OF QUANTITATIVE DETERMINATION OF NIFEDIPINE IN TABLETS
This article presents the results of the veriEication methods of quantitative determination of nifedipine in tablets. Validative characteristics of spectrophotometric methods of quantitative determination by means of the standard method: accuracy, convergence, accuracy, precision, linearity and robustness have been studied. These characteristics do not exceed the critical value of error (2.4 %) and are characterized by qualitative analytical indices. This technique can be correctly reproduced in the laboratory conditions.
Key words: veriEication; nifedipine; spectrophotometry.

V. Yu. Moskalenko, S. I. Merzlikin
ISOLATION OF METFORMIN FROM BIOLOGICAL OBJECTS
The dependence of the extraction degree of metformin from biological objects on isolation conditions, nature of the object and the method of quantitative determination of the drug in the extracts received has been researched. The most acceptable conditions of metformin isolating and biological objects for forensic toxicology research of unknown toxicant structure have been identi™ied.
Key words: metformin; isolation from the liver, urine and blood; extraction; UV-spectroscopy; highperformance liquid chromatography; extractive photometry.